Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 57, Issue 2, Pages 243-248Publisher
WILEY
DOI: 10.1002/mrm.21128
Keywords
biochemical validation; C-13 MRS; brain glycogen; metabolism; hypoglycemia
Funding
- NCRR NIH HHS [P41 RR08079, P41 RR008079] Funding Source: Medline
- NINDS NIH HHS [R01 NS42005, R01 NS042005, R01 NS042005-05] Funding Source: Medline
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With the use of localized C-13 MRS in conjunction with [1-C-13]-D-glucose infusion, it is possible to study brain glycogen metabolism in vivo. The purpose of this study was to validate in vivo 13C MRS measurements by comparing them with results from a standard biochemical assay. To increase the [1-C-13] glycogen concentration, 11 rats were subjected to an episode of acute hypoglycemia followed by a mild hyperglycemic recovery period during which [1-C-13]-D-glucose was infused. The total brain [1-C-13] glycogen of the same animal was determined from the enzymatically determined total brain glycogen content, which was fixed by focused microwave irradiation (4 kW in 1.4 s) immediately after the end of the in vivo NMR measurements. The corresponding isotopic enrichment (IE) of glycogen was measured by in vitro H-1 MRS of protons bound to glucose C1-alpha. The in vivo [1-C-13] glycogen concentration was strongly correlated to the in vitro [1-C-13] glycogen content determined by biochemical measurement in a linear manner (R = 0.79). The results are consistent with the notion that localized 13C MRS measurements closely reflect 13C glycogen content in the brain. Magn Reson Med 57:243-248, 2007. (c) 2007 Wiley-Liss, Inc.
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