Journal
JOURNAL OF NEUROPHYSIOLOGY
Volume 97, Issue 2, Pages 1058-1067Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.01007.2006
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- NHLBI NIH HHS [HL 074311] Funding Source: Medline
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The most common single-nucleotide polymorphism ( SNP) of the human mu-opioid receptor ( hMOR) gene occurs at position 118 ( A118G) and results in substitution of asparagine to aspartate at the N-terminus. The purpose of the present study was to compare the pharmacological profile of several opioid agonists to heterologously expressed hMOR and N- type Ca2+ channels in sympathetic neurons. cDNA constructs coding for wild-type and mutant hMOR were microinjected in rat superior cervical ganglion neurons and N- type Ca2+ channel modulation was investigated using the whole cell variant of the patch-clamp technique. Concentration - response relationships were generated with the following selective MOR agonists: DAMGO, morphine, morphine-6-glucuronide ( M-6-G), and endomorphin I. The estimated maximal inhibition for the agonists ranged from 52 to 64% for neurons expressing either hMOR subtype. The rank order of potencies for estimated EC50 values ( nM) in cells expressing wild-type hMOR was: DAMGO ( 31) >> morphine ( 76) congruent to M- 6- G ( 77) congruent to endomorphin I ( 86). On the other hand, the rank order in mutant-expressing neurons was: DAMGO ( 14) >> morphine ( 39) >> endomorphin I ( 74) congruent to M- 6- G ( 82), with a twofold leftward shift for both DAMGO and morphine. The DAMGO-mediated Ca2+ current inhibition was abolished by the selective MOR blocker, CTAP, and by pertussis toxin pretreatment of neurons expressing either hMOR subtype. These results suggest that the A118G variant MOR exhibits an altered signal transduction pathway and may help explain the variability of responses to opiates observed with carriers of the mutant allele.
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