Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 45, Issue 2, Pages 544-547Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01728-06
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Metallo-p-lactamase enzymes (MOL) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. The objective of this study was to develop a multiplex real-time PCR assay followed by a melt curve step for rapid detection and identification of genes encoding MOL-type enzymes based on the amplicon melting peak. The reference sequences of all genes encoding IMP and VIM types, SPM-1, GIM-1, and SIM-1 were downloaded from GenBank, and primers were designed to obtain amplicons showing different sizes and melting peak temperatures (T.). The real-time PCR assay was able to detect all M beta L-harboring clinical isolates, and the T-m-assigned genotypes were 100% coincident with previous sequencing results. This assay could be suitable for identification of MOL-producing gram-negative bacteria by molecular diagnostic laboratories.
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