Journal
HUMAN CELL
Volume 20, Issue 1, Pages 15-22Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1749-0774.2007.00024.x
Keywords
cell line; cisplatin; gastric cell; gastrin or somatostatin secretion; poorly differentiated adenocarcinoma
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We successfully established a spontaneously cisplatin-resistant tumor cell line (designated as IGSK- 1) derived from original gastric carcinoma. The patient was a 75-year-old Japanese woman. The histopathological diagnosis was gastric poorly differentiated adenocarcinoma accompanied with metastatic foci in lymph nodes, pT3, N2 MO, stage IIIB. The IGSK-1 cells grew as adhesive and monolayered cultures on the bottom of dishes. The susceptibility of the IGSK-1 cells to anti-cancer drugs was examined using oxygen electrode apparatus (Daikin, Tsukuba, JPN), and the results suggested TXL was effective, and CDDP, CPT-II and 5-FU were not effective. Gastrin and somatostatin secretions were confirmed by immunohistochemical staining and also radioimmunoassay. Immunohistochemistry and radioimmunoassay for serotonin suggested the IGSK-1 cells might incorporate serotonin from the growth media. Spontaneously cisplatin-resistant gastric carcinoma cell line secreted gastrin and somatostatin is very important material for chemotherapy.
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