Journal
JOURNAL OF BIOTECHNOLOGY
Volume 128, Issue 2, Pages 408-411Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2006.08.008
Keywords
non-natural amino acids; phenylalanine dehydrogenase; biocatalysis; cofactor recycling; chiral synthesis; site-directed mutagenesis
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Wild-type phenylalanine dehydrogenase from Bacillus sphaericus, and three mutants N145A, N145V and N145L, are used with a coenzyme recycling system to synthesise L-phenylalanine and three non-natural amino acids (p-F-phenylalanine, p-MeO-phenylalanine and p-CF3-phenylalanine) on a millimole scale. A range of reaction conditions are investigated. The kinetic parameters of WT PheDH and N145A towards p-CF3-phenylpyruvate are compared, emphasising the value of protein engineering in creating improved biocatalysts. (c) 2006 Elsevier B.V. All rights reserved.
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