Journal
BIOMATERIALS
Volume 28, Issue 6, Pages 936-946Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2006.09.012
Keywords
adipose stem cells; preadipocytes; cell adhesion; tissue engineering; ASC
Funding
- NIDDK NIH HHS [R01 DK054932, R56 DK054932, DK54932] Funding Source: Medline
- NIGMS NIH HHS [T32 GM08555, T32 GM008555] Funding Source: Medline
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Attachment of adipose-derived stem cells (ASCs) to biomaterials prior to implantation is a possible strategy for mediating inflammation and wound healing. In this study, the ASC percent coverage was measured on common medical grade biosensor materials subjected to different surface treatments. Cell coverage on silicone elastomer (poly-dimethylsiloxane) was below 20% for all surface treatments. Polyimide (Kapton), polyurethane (Pellethane) and tissue culture polystyrene all exhibited > 50% coverage for surfaces treated with fibronectin (Fn), fibronectin plus avidin/biotin (dual ligand), and oxygen plasma plus fibronectin treatments (FnO(2)). The fibronectin treatment performed as well or better on polyimide, polyurethane, and tissue culture polystyrene compared to the dual ligand and fibronectin oxygen plasma-treated surfaces. Cell detachment with increasing shear stresses was < 25% for each attachment method on both polyimide and polyurethane. The effects of attachment methods on the basic cell functions of proliferation, metabolism, ATP concentration, and caspase-3 activity were analyzed yielding proliferation profiles that were very similar among all of the materials. No significant differences in metabolism, intracellular ATP, or intracellular caspase-3 activity were observed for any of the attachment methods on either polyimide or polyurethane. (c) 2006 Elsevier Ltd. All rights reserved.
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