Journal
BIOTECHNIQUES
Volume 42, Issue 2, Pages 137-+Publisher
INFORMA HEALTHCARE
DOI: 10.2144/000112396
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We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology.
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