Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 57, Issue 47, Pages 15369-15373Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201808070
Keywords
antibodies; bioluminescence; paper-based analytical devices; point-of-care testing; whole-blood analysis
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Funding
- Japan Society for the Promotion of Science (JSPS)
- JSPS [18H02008]
- ERC [ERC-2011-StG 280255, ERC-2013-PoC 632274, ERC-2016-PoC 755471]
- Grants-in-Aid for Scientific Research [18H02008] Funding Source: KAKEN
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This work reports on fully integrated sample-insignal-out microfluidic paper-based analytical devices (mu PADs) relying on bioluminescence resonance energy transfer (BRET) switches for analyte recognition and colorimetric signal generation. The devices use BRET-based antibody sensing proteins integrated into vertically assembled layers of functionalized paper, and their design enables sample volume-independent and fully reagent-free operation, including ondevice blood plasma separation. User operation is limited to the application of a single drop (20-30 mu L) of sample (serum, whole blood) and the acquisition of a photograph 20 min after sample introduction, with no requirement for precise pipetting, liquid handling, or analytical equipment except for a camera. Simultaneous detection of three different antibodies (anti-HIV1, anti-HA, and anti-DEN1) in whole blood was achieved. Given its simplicity, this type of device is ideally suited for user-friendly point-of-care testing in low-resource environments.
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