Journal
MOLECULAR AND CELLULAR NEUROSCIENCE
Volume 34, Issue 2, Pages 209-218Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2006.10.015
Keywords
neurosecretory cells; diapause hormone; DH-PBAN; neuropeptide hormone; Pitx; bicoid-like homeobox; baculovirus; diapause; Bombyx mori; insect
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The diapause hormone-pheromone biosynthesis activating neuropeptide gene, DH-PBAN, is expressed exclusively in seven pairs of DH-PBAN-producing neurosecretory cells (DHPCs) on the terminally differentiated processes of the subesophageal ganglion (SG). To help reveal the regulatory mechanisms of cell-specific DH-PBAN expression, we identified a cis-regulatory element that regulates expression in DHPCs using the recombinant AcNPV-mediated gene transfer system and a gel-mobility shift assay. Bomhyx mori Pitx (BmPitx), a bicoid-like homeobox transcription factor, binds this element and activates DH-PBAN expression. The BmPitx was expressed in various tissues, including DHPCs in the SG. Suppression of DH-PBAN expression by silencing of the BmPitx successfully induced non-diapaused eggs from a diapause egg producer. To the best of our knowledge, this report is the first to identify, a neuropeptide-en coding gene as a target of the Pitx transcriptional regulator in invertebrates. Thus, it is tempting to speculate that functional conservation of Pitx family members on neuropeptide gene expression occurs through a combinational code mechanism in both vertebrate and invertebrate in neuroendocrine systems. (c) 2006 Elsevier Inc. All rights reserved.
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