Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 57, Issue 45, Pages 14942-14946Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201808666
Keywords
bacterial infections; biosensors; electrochemistry; metabolic activity; point-of-care testing
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Funding
- China Scholarship Council
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A combination of an immuno-affinity enrichment strategy and sensitive amperometric read-out was implemented in a point-of-care platform intended for bacterial infection analysis. Bacterial cells, selectively captured and enriched from complex matrices through immuno-affinity, were detected by amperometric monitoring of the redox state of metabolic activity indicators, providing species identification and viable-cell quantification. The method was successfully employed for the diagnosis of bacterial infections including antimicrobial susceptibility testing with only several hours of total working time.
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