Journal
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Volume 109, Issue 2, Pages 180-185Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ejlt.200600193
Keywords
assay; fat quality; immobilized lipase; lipase stability; microtiter plate
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A microtiter plate (MTP) method was developed to determine the quality of fats that are used in large-scale processing using lipase catalysis. Two assay formats were followed: In the first approach, the fats were interesterified with p-nitrophenol laurate using a lipase from Thermomyces lanuginosa; in the second approach, pH indicators were added to the fat samples containing lipase. A blind study using 29 fats showed that the MTP method using p-nitrophenol as pH indicator allowed a rapid and reliable assignment of bad fats and an acceptable differentiation between fats of moderate and good quality.
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