Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 31, Pages 8221-8224Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201404323
Keywords
antibodies; microarrays; micropatterning; hydrogels; single-cell detection
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Funding
- NIH [C4EB012836, DK073901]
- NSF [0937997]
- UC Davis
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Cells may be captured and released using a photodegradable hydrogel (photogel) functionalized with antibodies. Photogel substrates were used to first isolate human CD4 or CD8 T-cells from a heterogeneous cell suspension and then to release desired cells or groups of cells by UV-induced photodegradation. Flow cytometry analysis of the retrieved cells revealed approximately 95% purity of CD4 and CD8 T-cells, suggesting that this substrate had excellent specificity. To demonstrate the possibility of sorting cells according to their function, photogel substrates that were functionalized with anti-CD4 and anti-TNF-alpha antibodies were prepared. Single cells captured and stimulated on such substrates were identified by the fluorescence halo after immunofluorescent staining and could be retrieved by site-specific exposure to UV light through a microscope objective. Overall, it was demonstrated that functional photodegradable hydrogels enable the capture, analysis, and sorting of live cells.
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