Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 50, Pages 13734-13739Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201407606
Keywords
bacteria; biosensors; fluorescent probes; mathematical analysis
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Funding
- National Natural Science Foundation of China [21222502, 21025520, 20933008, 91213305]
- Ministry of Science and Technology of China [2013CB733700]
- CAS/SAFEA International Partnership Program for Creative Research Teams
- Beijing Natural Science Foundation [2122058]
- Youth Innovation Promotion Association (CAS)
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We report a method for the rapid and efficient identification of bacteria making use of five probes having fluorescent characteristics (F-array) and subsequent statistical analysis. Eight kinds of bacteria, including normal and multidrug-resistant bacteria, are differentiated successfully. Our easy-to-perform and time-saving method consists of mixing bacteria and probes, recording fluorescent intensity data by automated flow cytometry, and statistical analysis. No washing steps are required in order to identify the different bacteria simultaneously.
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