Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 7, Pages 2137-2141Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201410200
Keywords
anions; giant unilamellar vesicles; ion transport; membranes; supramolecular chemistry
Categories
Funding
- Engineering and Physical Sciences Research Council [EP/J00961X/1]
- European Cooperation in Science and Technology (COST) action Supramolecular Chemistry in Water (Short term scientific mission) [CM1005, COST-STSM-CM1005-16081]
- CONACyT
- ERC [240394]
- EPSRC [EP/J00961X/1, EP/F03623X/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/F03623X/1, EP/J00961X/1] Funding Source: researchfish
- European Research Council (ERC) [240394] Funding Source: European Research Council (ERC)
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Transmembrane ion transporters (ionophores) are widely investigated as supramolecular agents with potential for biological activity. Tests are usually performed in synthetic membranes that are assembled into large unilamellar vesicles (LUVs). However transport must be followed through bulk properties of the vesicle suspension, because LUVs are too small for individual study. An alternative approach is described whereby ion transport can be revealed and quantified through direct observation. The method employs giant unilamellar vesicles (GUVs), which are 20-60 mm in diameter and readily imaged by light microscopy. This allows characterization of individual GUVs containing transporter molecules, followed by studies of transport through fluorescence emission from encapsulated indicators. The method provides new levels of certainty and relevance, given that the GUVs are similar in size to living cells. It has been demonstrated using a highly active anion carrier, and should aid the development of compounds for treating channelopathies such as cystic fibrosis.
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