Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 31, Pages 8066-8069Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201402327
Keywords
biochemical networks; biosensors; enzyme catalysis; reaction-diffusion systems; ultrasensitivity
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Funding
- European Research Council (ERC) [246812]
- Netherlands Organisation for Scientific Research (NWO
- VENI) [722.012.001]
- Netherlands Organisation for Scientific Research (NWO
- VICI) [700.10.44]
- Netherlands Organisation for Scientific Research (NWO
- Marie Curie Intra-European Fellowship) [300519]
- Ministry of Education, Culture and Science [024.001.035]
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A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks.
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