Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 38, Pages 10114-10118Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201405355
Keywords
antisense agents; cage compounds; developmental biology; gene expression; oligonucleotides
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Funding
- NIH [R01 GM087292, DP1 HD075622, R01 GM079114]
- Japan Society for the Promotion of Science Fellowship
- A.P. Giannini Foundation Fellowship for Medical Research
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Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength-selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse-genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning.
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