Journal
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
Volume 35, Issue 2, Pages 61-67Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bse.2006.05.020
Keywords
Scolytidae; Hylastinus obscurus; volatiles; red clover; Trifolium pratense
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Root extracts from 1.5 and 2.5-year-old red clover (Trifolium pratense) were obtained using supercritical fluid extraction (SFE). GC-MS analysis and Kovats indices allowed identification of the volatile compounds as butyl acetate, E-2-hexenal, alpha-pinene, benzaldehyde, 6-methyl-5-hepten-2-one, limonene, acetophenone, methyl benzoate, nonanal, octanoic acid and decanal. The extracts obtained and pure synthetic samples of the identified volatile components were tested in olfactometric bioassays with the insect Hylastinus obscurus (Coleoptera: Scolytidae). The results showed that the insect was attracted to root volatiles of 1.5-year-old extracts but not to those from 2.5-year-old extracts. A 10 mu g dose of methyl benzoate and E-2-hexenal attracted the insect, whereas the same dose of limonene repelled the red clover borer. An increase in limonene content and a decrease in E-2-hexenal content were correlated with a loss of attraction to 2.5-year-old clover roots. (c) 2006 Elsevier Ltd. All rights reserved.
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