Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 47, Pages 12735-12740Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201405991
Keywords
DNA nanotechnology; DNA origami; molecular crowding; poly(ethylene glycol); self-assembly
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Funding
- Deutsche Forschungsgemeinschaft through the Excellence Cluster Center for Integrated Protein Science, Nano Initiative Munich
- TUM International Graduate School of Science and Engineering (IGSSE)
- European Research Council Starting Grant [256270]
- [Sonderforschungsbereich SFB863]
- European Research Council (ERC) [256270] Funding Source: European Research Council (ERC)
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DNA has become a prime material for assembling complex three-dimensional objects that promise utility in various areas of application. However, achieving user-defined goals with DNA objects has been hampered by the difficulty to prepare them at arbitrary concentrations and in user-defined solution conditions. Here, we describe a method that solves this problem. The method is based on poly(ethylene glycol)induced depletion of species with high molecular weight. We demonstrate that our method is applicable to a wide spectrum of DNA shapes and that it achieves excellent recovery yields of target objects up to 97%, while providing efficient separation from non-integrated DNA strands. DNA objects may be prepared at concentrations up to the limit of solubility, including the possibility for bringing DNA objects into a solid phase. Due to the fidelity and simplicity of our method we anticipate that it will help to catalyze the development of new types of applications that use self-assembled DNA objects.
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