Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 7, Pages 2151-2155Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201410720
Keywords
biosensors; DNA nanostructures; electrochemistry; soft lithography; surface chemistry
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Funding
- National Basic Research Program (973 Program) [2012CB932600, 2013CB932803]
- NSFC [91313302, 21390414, 21422508, 21329501]
- Shanghai Pujiang Project [13PJ1410700]
- Chinese Academy of Sciences
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Self-assembled DNA nanostructures with precise sizes allow a programmable soft lithography approach to engineer the interface of electrochemical DNA sensors. By using millimeter-sized gold electrodes modified with several types of tetrahedral DNA nanostructures (TDNs) of different sizes, both the kinetics and thermodynamics of DNA hybridization were profoundly affected. Because each DNA probe is anchored on an individual TDN, its lateral spacing and interactions are finely tuned by the TDN size. By simply varying the size of the TDNs, the hybridization time was decreased and the hybridization efficiency was increased. More significantly, the detection limit for DNA detection was tuned over four orders of magnitude with differentially nanostructured electrodes, and achieved attomolar sensitivity with polymeric enzyme amplification.
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