Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 7, Pages 1845-1848Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201307418
Keywords
bioanalysis; DNA structures; hairpin loops; mismatch; nanobiotechnology
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Funding
- Bill and Melinda Gates Foundation [OPP1028808]
- Defense Advanced Research Projects Agency [HR0011-12-2-0001, 5-35830]
- National Institute of Health [TR01 (5 R01 AI092839)]
- Bill and Melinda Gates Foundation [OPP1028808] Funding Source: Bill and Melinda Gates Foundation
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Catalytic hairpin assembly (CHA) has previously proven useful as a transduction and amplification method for nucleic acid detection. However, the two hairpin substrates in a CHA circuit can potentially react non-specifically even in the absence of a single-stranded catalyst, and this non-specific background degrades the signal-to-noise ratio. The introduction of mismatched base pairs that impede uncatalyzed strand exchange reactions led to a significant decrease of the background signal, while only partially damping the signal in the presence of a catalyst. Various types and lengths of mismatches were assayed by fluorimetry, and in many instances, our MismatCHA designs yielded 100-fold increased signal-to-background ratios compared to a ratio of 4:1 with the perfectly matched substrates. These observations could be of general utility for the design of non-enzymatic nucleic acid circuits.
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