Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 45, Pages 12137-12141Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201407468
Keywords
autophagy; bioinorganic chemistry; iridium; probe; theranostic
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Funding
- National Natural Science Foundation of China [21172274, 21231007, 21121061, 21201183]
- 973 Program [2014CB845604]
- 863 Program [2012AA020305]
- Ministry of Education of China [IRT1298, 313058]
- Fundamental Research Funds for the Central Universities
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During autophagy, the intracellular components are captured in autophagosomes and delivered to lysosomes for degradation and recycling. Changes in lysosomal trafficking and contents are key events in the regulation of autophagy, which has been implicated in many physiological and pathological processes. In this work, two iridium(III) complexes (LysoIr1 and LysoIr2) are developed as theranostic agents to monitor autophagic lysosomes. These complexes display lysosome-activated phosphorescence and can specifically label lysosomes with high photostability. Simultaneously, they can induce autophagy potently without initiating an apoptosis response. We demonstrate that LysoIr2 can effectively implement two functions, namely autophagy induction and lysosomal tracking, in the visualization of autophagosomal-lysosomal fusion. More importantly, they display strong two-photon excited fluorescence (TPEF), which is favorable for live cell imaging and in vivo applications.
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