Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 52, Pages 14517-14521Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201407824
Keywords
antibodies; arginine; glycopeptides; N-GlcNAcylation; solid-phase peptide synthesis
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Funding
- National Basic Research Program of China (973 program) [2013CB932800]
- National Major Project of China [2012ZX09J12108-01, 2012ZX09502-001-005]
- NSFC [91313301, 31470245]
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As a unique and unappreciated protein posttranslational modification, arginine N-glycosylation was recently discovered to play an important role in the process that bacteria counteract host defenses. To provide chemical tools for further proteomic and biochemical studies on arginine N-glycosylation, we report the first general strategy for a rapid and cost-effective synthesis of glycopeptides carrying single or multiple arginine N-GlcNAcyl groups. These glycopeptides were successfully utilized to generate the first antibodies that can specifically recognize arginine N-GlcNAcylated peptides or proteins in a sequence-independent manner.
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