Journal
JOURNAL OF LIPID RESEARCH
Volume 48, Issue 2, Pages 417-424Publisher
ELSEVIER
DOI: 10.1194/jlr.M600344-JLR200
Keywords
molecular species; lipidomics; mass spectrometry; lipid rafts
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The ceramide (Cer) and sphingomyelin (SM) species of cultured differentiated rat cerebellar granule cells and human fibroblasts were characterized by electrospray ionization-mass spectrometry. We identified 35 different species of Cer and 18 species of SM in human fibroblasts, and 35 different species of Cer and 9 species of SM were characterized in rat neurons. The main Cer species of rat cerebellar granule cells contained d18:1 sphingosine linked with palmitic, stearic, or nervonic fatty acid, and the two main SM species were d18: 1,16:0 and d18: 1,18:0. Both sphin-golipids were enriched in detergent-resistant membranes (DRMs; or lipid rafts), and significant differences were found in the sphingolipid patterns of DRMs and of detergent-soluble fractions (DSF) from these cells. In human fibroblasts, the main Cer species were d18: 1,16: 0, d18: 2,16: 0, d18: 1,24: 0, d18: 2,24:0, d18: 1,24: 1, and d18: 2,24: 1; the most represented species of SM were d18: 1,16: 0, d18: 1,24: 0, and d18: 1,24:1. In these cells, SM was highly enriched in DRMs and Cer was mainly associated with DSF, and the species found in DRMs were markedly different from those found in DSF. - Valsecchi, M., L. Mauri, R. Casellato, S. Prioni, N. Loberto, A. Prinetti, V. Chigorno, and S. Sonnino. Ceramide and sphingomyelin species of fibroblasts and neurons in culture.
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