Journal
PROTEIN SCIENCE
Volume 16, Issue 2, Pages 239-249Publisher
WILEY-BLACKWELL
DOI: 10.1110/ps.062538707
Keywords
enzymes; computational analysis of protein structure; pH-activity profile; pKa calculations; protein electrostatics
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The ability to re-engineer enzymatic pH-activity profiles is of importance for industrial applications of enzymes. We theoretically explore the feasibility of re-engineering enzymatic pH-activity profiles by changing active site pK(a) values using point mutations. We calculate the maximum achievable Delta pK(a) values for 141 target titratable groups in seven enzymes by introducing conservative net-charge altering point mutations. We examine the importance of the number of mutations introduced, their distance from the target titratable group, and the characteristics of the target group itself. The results show that multiple mutations at 10 angstrom can change pK(a) values up to two units, but that the introduction of a requirement to keep other pK(a) values constant reduces the magnitude of the achievable Delta pK(a). The algorithm presented shows a good correlation with existing experimental data and is available for download and via a web server at http://enzyme.ucd.ie/pKD.
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