Synthetic Disialyl Hexasaccharides Protect Neonatal Rats from Necrotizing Enterocolitis

Two novel synthetic alpha 2-6-linked disialyl hexasaccharides, disialyllacto-N-neotetraose (DS'LNnT) and alpha 2-6-linked disialyllacto-N-tetraose (DS'LNT), were readily obtained by highly efficient one-pot multienzyme (OPME) reactions. The sequential OPME systems described herein allowed the use of an inexpensive disaccharide and simple monosaccharides to synthesize the desired complex oligosaccharides with high efficiency and selectivity. DSLNnT and DS'LNT were shown to protect neonatal rats from necrotizing enterocolitis (NEC) and are good therapeutic candidates for preclinical experiments and clinical application in treating NEC in preterm infants.