Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 57, Issue 2, Pages 163-167Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2006.08.009
Keywords
Enterocytozoon bieneusi; Encephalitozoon spp.; real-time PCR diarrhea
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A multiplex real-time polymerase chain reaction (PCR) method was developed for the simultaneous detection of Enteroclytozoon bieneusi (n = 30) and Encephalitozoon spp. (n = 3) in stool samples. The multiplex PCR also included an internal control to detect inhibition of the amplification by fecal constituents in the sample. The assay was performed on species-specific DNA controls (n = 22) and a range of well-defined stool samples (n = 140), and it achieved 100% specificity and sensitivity. The use of this assay in a diagnostic laboratory offers the possibility of introducing DNA detection as a feasible technique in the routine diagnosis of intestinal microsporidian infections. (c) 2007 Elsevier Inc. All rights reserved.
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