Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 31, Pages 8137-8141Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201404043
Keywords
DNA nanotechnology; DNA origami nanostructures; mechanochemical sensing; optical tweezers; single-molecule techniques
Categories
Funding
- NSF [CHE-1026532]
- JST CREST [24310097, 24104002, 25253004]
- JSPS KAKENHI [24310097, 24104002, 25253004]
- Grants-in-Aid for Scientific Research [25253004, 24104002, 26620133] Funding Source: KAKEN
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While single-molecule sensing offers the ultimate detection limit, its throughput is often restricted as sensing events are carried out one at a time in most cases. 2D and 3D DNA origami nanostructures are used as expanded single-molecule platforms in a new mechanochemical sensing strategy. As a proof of concept, six sensing probes are incorporated in a 7-tile DNA origami nanoassembly, wherein binding of a target molecule to any of these probes leads to mechanochemical rearrangement of the origami nanostructure, which is monitored in real time by optical tweezers. Using these platforms, 10 pm platelet-derived growth factor (PDGF) are detected within 10 minutes, while demonstrating multiplex sensing of the PDGF and a target DNA in the same solution. By tapping into the rapid development of versatile DNA origami nanostructures, this mechanochemical platform is anticipated to offer a long sought solution for single-molecule sensing with improved throughput.
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