Journal
PEPTIDES
Volume 28, Issue 2, Pages 226-234Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2006.08.042
Keywords
Y receptors; live cell imaging; GPCR trafficking; fluorescent tag; EYFP; Lumio (TM)-tag; SNAP (TM)-tag
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Non-invasive methods for studying biological processes in living cells have become very important, also in the field of GPCR biochemistry. Great advancements in the application of fluorescence techniques as well as in the development and improvement of novel fluorophores allow the visualization of dynamic processes. Using these technologies, problems concerning receptor biosynthesis, internalization, recycling and degradation can be investigated. Here we compare the application of the different fluorescent tags EYFP, Lumio (TM) and SNAP (TM) to track hY(1) and hY(5) receptors in living cells. (c) 2006 Elsevier Inc. All rights reserved.
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