Journal
CELL AND TISSUE RESEARCH
Volume 327, Issue 2, Pages 301-311Publisher
SPRINGER
DOI: 10.1007/s00441-006-0257-6
Keywords
dental follicle; progenitor; development; immortalization; periodontal ligament; mouse (ICR)
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The dental follicle is a mesenchymal tissue that surrounds the developing tooth germ. During tooth root formation, periodontal components, viz., cementum, periodontal ligament (PDL), and alveolar bone, are created by dental follicle progenitors. Here, we report the presence of PDL progenitors in mouse dental follicle (MDF) cells. MDF cells were obtained from mouse incisor tooth germs and immortalized by the expression of a mutant human papilloma virus type 16 E6 gene lacking the PDZ-domain-binding motif. MDF cells expressing the mutant E6 gene (MDFE6-EGFP supercript stop cells) had an extended life span, beyond 150 population doublings (PD). In contrast, normal MDF cells failed to proliferate beyond 10 PD. MDFE6-EGFP supercript stop cells expressed tendon/ligament phenotype-related genes such as Scleraxis (Scx), growth and differentiation factor-5, EphA4, Six-1, and type I collagen. In addition, the expression of periostin was observed. To elucidate the differentiation capacity of MDFE6-EGFP supercript stop cells in vivo, the cells were transplanted into severe combined immunodeficiency mice. At 4 weeks, MDFE6-EGFP supercript stop cell transplants had the capacity to generate a PDL-like tissue that expressed periostin, Scx, and type XII collagen and the fibrillar assembly of type I collagen. Our findings suggest that MDFE6-EGFP supercript stop cells can act as PDL progenitors, and that these cells may be a useful research tool for studying PDL formation and for developing regeneration therapies.
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