Genistein attenuates postischemic ovarian injury in a rat adnexal torsion-detorsion model

Objective: To investigate the effects of genistein on reperfusion injury in a rat ovarian torsion-detorsion model. Design: Controlled experimental study. Setting: University animal research laboratory. Subject(s): Thirty-two Wistar-Albino rats. Intervention(s): The rats were divided into four groups. Sham operation was performed in group I. In group II, 5 mg/kg genistein was given intraperitoneally (IP) during laparotomy, and right ovaries were removed 4 hours later. In group III, right ovaries were subjected to 4 hours of adnexal ischemic by use of vascular clips, and after inchemic insult, 4 hours of reperfusion was maintained by removing the clips. In group IV, after the ischemic period, 5 mg/kg genistein was given IP, and 4 hours of reperfusionwas maintained. Right ovaries were surgically removed at the end of the procedure in each group. Main Outcome Measures(s): Ovarian histopathologic findinf were scored and compared among study groups. Serum and ovarian tissue malondialdehyde (MDA), glutathione peroxidase, and superoxide dismutase, levels were measured. Result(s): Ovarian tissue damage scores were significantly decreased the tissue damage scores, ovarian tissue MDA levels, and serum MDA levels. Conclusion(s): Genistein attenuates ischemia-reperfusion injury in rat adnexal torsion-detorsion model.