Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 184, Issue 2, Pages 344-349Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2006.11.002
Keywords
in vivo MRS; magnetization transfer; malate dehydrogenase; carbon-13; enzymology
Funding
- Intramural NIH HHS [Z01 MH002803-06] Funding Source: Medline
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Malate dehydrogenase catalyzes rapid interconversion between dilute metabolites oxaloacetate and malate. Both oxaloacetate and malate are below the detection threshold of in vivo MRS. Oxaloacetate is also in rapid exchange with aspartate catalyzed by aspartate aminotransferase, the latter metabolite is observable in vivo using C-13 MRS. We hypothesized that the rapid turnover of oxaloacetate can effectively relay perturbation of magnetization between malate and aspartate. Here, we report indirect observation of the malate dehydrogenase reaction by saturating malate C2 resonance at 71.2 ppm and detecting a reduced aspartate C2 signal at 53.2 ppm due to relayed magnetization transfer via oxaloacetate C2 at 201.3 ppm. Using this strategy the rate of the cerebral malate clehydrogenase reaction was determined to be 9 +/- 2 mu mol/g wet weight/min (means +/- SD, n = 5) at 11.7 Tesla in anesthetized adult rats infused with [1,6- C-13(2)]glucose. Published by Elsevier Inc.
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