4.8 Article

Real-Time Monitoring of Auxin Vesicular Exocytotic Efflux from Single Plant Protoplasts by Amperometry at Microelectrodes Decorated with Nanowires

Journal

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 10, Pages 2643-2647

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201308972

Keywords

amperometric monitoring; auxin efflux; electrochemical sensors; exocytosis; single plant cells

Funding

  1. National Natural Science Foundation of China [91017013, 31070995, 21375099]
  2. Program for Changjiang Scholars and Innovative Research Team in University [IRT1030]
  3. UMR (CNRS-ENS-UPMC)
  4. UMR LIA CNRS XiamENS

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Recent biochemical results suggest that auxin (IAA) efflux is mediated by a vesicular cycling mechanism, but no direct detection of vesicular IAA release from single plant cells in real-time has been possible up to now. A TiC@C/Pt-QANFA micro-electrochemical sensor has been developed with high sensitivity in detection of IAA, and it allows real-time monitoring and quantification of the quantal release of auxin from single plant protoplast by exocytosis.

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