Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1141, Issue 1, Pages 131-137Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.12.008
Keywords
diazepam; nordazepam; oxazepam; gas chromatography; mass spectrometry; electron capture ionization
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A gas chromatography-tandem mass spectrometry method for detection of diazepam, nordazepam and oxazepam is presented. The method associates electron capture ionization and multiple reaction monitoring (MRM). No derivatization is performed; oxazepam undergoes thermal degradation during chromatographic injection and is thus quantified via its decomposition product. The negative molecular ions are so stable that they do not dissociate when collision is performed under classical conditions (i.e. with argon as collision gas). With xenon as collision gas, the energy transfer is sufficient to provide two product ions for diazepam and nordazepam and one product ion for the decomposition product of oxazepam. The sample preparation part involves liquid/liquid extraction with TOXI-TUBES (R) A extraction tubes; it provides recovery yields between 68 and 95%, depending of the benzodiazepine considered, with coefficients of variation below 6% for 10 samples. The applicability of the method was demonstrated on urine extracts. From 1 mL of urine, the method provides quantitation limits of 0.15 ng/mL for diazepam, 1.0 ng/mL for nordazepam and 1.5 ng/mL for oxazepam. Mechanisms of dissociation of M center dot- ions of benzodiazepines are suggested. (c) 2006 Elsevier B.V. All rights reserved.
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