Journal
TOXICOLOGY LETTERS
Volume 168, Issue 3, Pages 310-318Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2006.10.028
Keywords
arsenic; atomic fluorescence spectrometry; inductively coupled plasma mass spectrometry; atomic absorption spectrometry; urine samples
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This work compares the three most common analytical methods for determination of inorganic arsenic and its metabolites in urine: high performance liquid chromatography coupled to either inductively coupled plasma mass spectrometry or atomic fluorescence spectrometry via hydride generation (high performance liquid chromatography-hydride generation-inductively coupled plasma mass spectrometry (HPLC-HG-ICPMS) and HPLC-HG-atomic fluorescence spectrometry (AFS), respectively) and atomic absorption spectrometry coupled to HG (HG-atomic absorption spectrometry (AAS)). This was done with the focus to find alternatives to ICPMS, the investment and running costs of which are rather high. Between-laboratory comparison of HPLC-HG-ICPMS and HPLC-HG-AFS showed good agreement for inorganic arsenic, methylarsonate (MA) and dimethylarsinate (DMA) (R-2=0.91, R-2=0.92 and R-2=0.90, respectively, N=86). Within-laboratory comparisons of HPLC-HG-AFS, HPLC-HG-ICPMS and HG-AAS showed good agreement for all arsenic species and the sum of inorganic arsenic and its metabolites in urine (HPLC-HG-ICPMS versus HPLC-HG-AFS: R-2=0.95; HG-AAS versus HPLC-HG-AFS: R-2=0.95 and HPLC-HG-ICPMS versus HG-AAS: R-2=0.97; N=89). HPLC-HG-AFS was found to be a simple, but high quality alternative to HPLC-HG-ICPMS for the speciation and quantification of inorganic arsenic and its metabolites in urine at arsenic concentrations above 10 mu g L-1. Because of its considerably lower costs compared to HPLC-HG-ICPMS, it may be a good alternative in laboratories where the high cost of ICPMS is notjustified in relation to the intended use of the instrument. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
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