Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 3, Pages 1036-1040Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201410223
Keywords
biogenic vesicles; cell-derived microparticles; nanoparticles; quantum dots; targeted delivery
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Funding
- National Basic Research Program of China (973 Program) [2011CB933600]
- National Natural Science Foundation of China [81300895, 81170977, 81371159]
- Doctoral Program Foundation of Higher Education of China [20130141120089, 20130141130006]
- China Postdoctoral Science Foundation [2013M540607]
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Cell-derived microparticles (MPs) have been recently recognized as critical intercellular information conveyors. However, further understanding of their biological behavior and potential application has been hampered by the limitations of current labeling techniques. Herein, a universal donor-cell-assisted membrane biotinylation strategy was proposed for labeling MPs by skillfully utilizing the natural membrane phospholipid exchange of their donor cells. This innovative strategy conveniently led to specific, efficient, reproducible, and biocompatible quantum dot (QD) labeling of MPs, thereby reliably conferring valuable traceability on MPs. By further loading with small interference RNA, QD-labeled MPs that had inherent cell-targeting and biomolecule-conveying ability were successfully employed for combined bioimaging and tumor-targeted therapy. This study provides the first reliable and biofriendly strategy for transforming biogenic MPs into functionalized nanovectors.
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