Journal
VACCINE
Volume 25, Issue 8, Pages 1444-1451Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2006.10.039
Keywords
autoimmune diabetes; CTB-insulin B chain; oral tolerance
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Oral tolerance has been applied successfully as a potential therapeutic strategy for preventing and treating autoimmune diseases including type I diabetes. In this paper we constructed an edible vaccine consisting of a fusion protein composed of cholera toxin B subunit (CTB) and insulin B chain (InsB) that was produced in silkworm larvae. The silkworm larvae produced this fusion protein at levels of up to 0.97 mg/ml of hemolymph as the pentameric CTB-InsB form, which retained the GM1-ganglioside binding affinity and the native antigenicity of CTB. Non-obese diabetic mice fed hemolymph containing microgram quantities of the CTB-InsB fusion protein showed a prominent reduction in pancreatic islet inflammation and a delay in the development of diabetic symptoms. This study demonstrates that silkworm-produced CTB-InsB fusion protein can be used as an ideal oral protein vaccine for induction of immunological tolerance against autoimmune diabetes. (c) 2006 Elsevier Ltd. All rights reserved.
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