Journal
ANALYTICA CHIMICA ACTA
Volume 584, Issue 1, Pages 160-165Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2006.11.023
Keywords
enantioseparation; levofloxacin; impurity; ligand-exchange chromatography
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Funding
- National Research Foundation of Korea [R11-2000-068-04003-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A sensitive, simple, and accurate method for determination of levofloxacin and its (R)-enantiomer was developed to determine the chiral impurity of levofloxacin in Cravit Tablets material by ligand-exchange high performance liquid chromatography. The effects of different kinds of ligands, concentration of ligands in mobile phase, organic modifier, pH of mobile phase, and temperature on enantioseparation were investigated and evaluated. Chiral separation was performed on a conventional C-18 column, where the mobile phase consisted of a methanol-water solution (containing10 mmol L-1 L-leucine and 5 mmol L-1 copper sulfate) (88:12, v/v) and its flow-rate was set at 1.0 mL min(-1). The conventional C-18 column offers baseline separation of two enantionters with a resolution of 2.4 in less than 20 min. Thermodynamic data (Delta Delta H and Delta Delta S) obtained by Van't Hoff plots revealed the chiral separation is an enthalpy-controlled process. The standard curves showed excellent linearity over the concentration range from 0.5 to 400 mg L-1 for levofloxacin and its (R)-enantiomer. The linear correlation equations are: y = 1.33 x 10(5)x + 6297 (r = 0.9991) and y = 1.34 x 10(5) x + 3565 (r = 0.9997), respectively. The relative standard deviation (RSD) of the method was below 2.3% (n = 3). (c) 2006 Elsevier B.V. All rights reserved.
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