Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 17, Issue 4, Pages 1037-1042Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2006.11.028
Keywords
carbonic anhydrase; secreted isoform; anion; enzyme inhibitor; cyanide; anion exchanger
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The unique secretory isozyme of human carbonic anhydrase (hCA, EC 4.2.1.1), hCA VI, has been cloned, expressed, and . The kinetic parameters for the CO2 hydration reaction proved hCA VI to possess a k(cat) of 3.4 x 10(5) s(-1) and k(cat)/K-M of 4.9 x 10(7) M-1 s(-1) (at pH 7.5 and 20 degrees C). hCA VI has a significant catalytic activity for the physiological reaction, of the same order of magnitude as isoforms CA I or CA IX. A series of anions (such as bicarbonate, chloride, nitrate, etc.) were shown to inhibit the activity of the enzyme, with inhibition constants typically in the range of 0.60-0.90 mM. The best hCA VI inhibitors were cyanide, azide sulfamide, and sulfamate, with inhibition constants in the range of 70-90 mu M. (c) 2006 Elsevier Ltd. All rights reserved.
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