Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 30, Pages 7765-7769Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201402863
Keywords
conformation analysis; immunoglobulin G; mass spectrometry; molecular dynamics; protein structures
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Funding
- MRC Industrial Case Studentship
- UCB Pharma
- Biotechnology and Biological Sciences Research Council [BB/L002655/1] Funding Source: researchfish
- Medical Research Council [1008655] Funding Source: researchfish
- BBSRC [BB/L002655/1] Funding Source: UKRI
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Collision cross-sections (CCS) of immunoglobulins G1 and G4 have been determined using linear drift-tube ion-mobility mass spectrometry. Intact antibodies and Fc-hinge fragments present with a larger range of CCS than proteins of comparable size. This is rationalized with MD simulations, which indicate significant in vacuo dynamics between linked folded domains. The IgG4 subclass presents over a wider CCS range than the IgG1 subclass.
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