Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 353, Issue 3, Pages 661-664Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2006.12.097
Keywords
SLUG; CtBP1; yeast 2-hybrid; co-immunoprecipitation; repressor; SNAG domain
Categories
Funding
- NCI NIH HHS [U54 CA091408-01, U54 CA091408, 1U54CA091408-010003] Funding Source: Medline
- NHLBI NIH HHS [T32 HL007737, 5 T32 HL007737] Funding Source: Medline
- NIMHD NIH HHS [U54 MD007593] Funding Source: Medline
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SLUG is a transcriptional repressor protein implicated to have major role in the oncogenesis and metastasis of human breast cells. We previously have shown by chromatin immunoprecipitation assay that human SLUG (hSLUG) is co-localized with the co-repressor protein CtBP1 as bound to the BRCA2 gene silencer [M.K. Tripathi, S. Misra, SN. Khedkar, N. Hamilton, C. Irvin-Wilson,, C. Sharan, L. Sealy, G. Chaudhuri, J. Biol. Chem. 280 (2005) 17163-17171]. hSLUG was predicted to be binding directly to CtBP1 because of an apparent presence of CtBP1 binding site in its amino acid sequences. Here, we provide evidence through yeast two-hybrid and in vitro co-immunoprecipitation analyses that hSLUG does not directly interacts with hCtBP1. This observation will help in the study of the mode of action of hSLUG in human cells. (c) 2006 Elsevier Inc. All rights reserved.
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