Journal
JOURNAL OF NEUROSCIENCE
Volume 27, Issue 8, Pages 2145-2149Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4641-06.2007
Keywords
GABAergic neurons; orientation selectivity; visual cortex; two-photon functional calcium imaging; transgenic mice; green fluorescent protein
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Most neurons in the visual cortex are selectively responsive to visual stimulation of a narrow range of orientations, and GABAergic neurons are considered to play a role in the formation of such orientation selectivity. This suggests that response properties of GABAergic neurons may be different from those of excitatory neurons. This view remains unproved, however. To address this issue, we applied in vivo two- photon functional Ca2+ imaging to transgenic mice, in which GABAergic neurons express enhanced green fluorescent protein. Astroglia were stained by an astrocyte- specific dye. The three types of cells, GABAergic neurons, excitatory neurons, and astrocytes, in layer II/ III of the visual cortex were differentially identified by using different wavelengths of excitation light and a dichroic mirror for emitted fluorescence, and their responses to moving visual stimuli at different orientations were measured with changes in the intensity of fluorescence of a Ca2+-sensitive dye. We found that almost all GABAergic neurons have orientation- insensitive responses, whereas most of excitatory neurons have orientation- selective responses.
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