Journal
MOLECULAR CELL
Volume 25, Issue 4, Pages 505-517Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2007.01.022
Keywords
-
Categories
Funding
- NIGMS NIH HHS [1R01GM079238-01, R01 GM079238] Funding Source: Medline
Ask authors/readers for more resources
High spatial and time resolution single-molecule fluorescence resonance energy transfer measurements have been used to probe the structural and kinetic parameters of transfer RNA (tRNA) movements within the aminoacyl (A) and peptidyl (P) sites of the ribosome. Our investigation of tRNA motions, quantified on wild-type, mutant, and L1-depleted ribosome complexes, reveals a dynamic exchange between three metastable tRNA configurations, one of which is a previously unidentified hybrid state in which only deacylated-tRNA adopts its hybrid (P/E) configuration. This new dynamic information suggests a framework in which the formation of intermediate states in the translocation process is achieved through global conformational rearrangements of the ribosome particle.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available