Journal
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Volume 70, Issue 1, Pages 3-13Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbbm.2006.11.002
Keywords
polyacrylamide monoliths; analytical glycobiology; capillary electrochromatography; branched oligosaccharides
Funding
- NIGMS NIH HHS [R01 GM024349, GM24349] Funding Source: Medline
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Capillary electrochromatography (CEC) of oligosaccharides in porous polyacrylamide monoliths has been explored. While it is possible to alter separation capacity for various compounds by copolymerization of suitable separation ligands in the polymerization backbone, blank acrylamide matrix is also capable of sufficient resolution of oligosaccharides in the hydrophilic interaction mode. The blank acrylamide network, formed with a more rigid crosslinker, provides maximum efficiency for separations (routinely up to 350,000 theoretical plates/m for fluorescently-labeled oligosaccharides). These columns yield a high spatial resolution of the branched glycan isomers and large column permeabilities. From the structural point of view, some voids are observable in the monoliths at the mesoporous range (mean pore radius ca. 35 nm, surface area of 74 m(2)/g), as measured by intrusion porosimetry in the dry state. (c) 2006 Elsevier B.V. All rights reserved.
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