Journal
ACTA CYTOLOGICA
Volume 51, Issue 2, Pages 193-196Publisher
KARGER
DOI: 10.1159/000325714
Keywords
uterine cervical cancer; human papillomavirus; thin-layer cytology; atypical squamous cells of undetermined significance
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Objective To ensure complete reliability in the detection of hyman papillomavirus using either Specimen Transport Medium (Digene Inc., Gaithersburg, Mmyland, U.S.A) or an alcoholic cell-preservative. Study Design In order to compare both media, we adjusted the cell content of specimens and optimized the denaturation duration to avoid chemiluminescence inhibition. Results We validated this protocol in 2 groups of 90 patients, leading to agreement of 100% in both positive and negative tests. This was confirmed using linear regression curves. We demonstrated that any change in the Digene protocol, especially the use of other preservatives, should be carefully evaluated since it may interfere with DNA denaturation and hybridization and the chemiluminescence signal. The main risk was obtaining false positive responses from nonspecific DNA hybridization and false negative ones because of chemiluminescence signal inhibition. Conclusion It is necessary to take into account preservative characteristics when a new protocol is under validation.
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