Journal
EUKARYOTIC CELL
Volume 6, Issue 3, Pages 563-567Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/EC.00301-06
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Protoplast and spheroplast preparations allow the transfer of macromolecules into cells and provide the basis for the generation of engineered organisms. Crypthecodinium cohnii cells harvested from polyethylene glycol-containing agar plates possessed significantly lower levels of cellulose in their cortical layers, which facilitated the delivery of fluorescence-labeled oligonucleotides into these cells.
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