Journal
JOURNAL OF VIROLOGY
Volume 81, Issue 5, Pages 2138-2148Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.02318-06
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Funding
- NIAID NIH HHS [R01 AI063987, P30 AI060354, AI 063987, AI 60354] Funding Source: Medline
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The host cell factors TRIM5 alpha(hu) and Fv-1 restrict N-tropic murine leukemia virus (N-MLV) infection at an early postentry step before or after reverse transcription, respectively. Interestingly, the identity of residue 110 of the MLV capsid determines susceptibility to both TRIM5 alpha(hu) and Fv-1. In this study, we investigate the fate of the MLV capsid in cells expressing either the TRIM5 alpha(hu) or Fv-1 restriction factor. The expression of TRIM5 alpha(hu), but not Fv-1, specifically promoted the premature conversion of particulate N-MLV capsids within infected cells to soluble capsid proteins. The TRIM5 alpha(hu)-mediated disassembly of particulate N-MLV capsids was dependent upon residue 110 of the viral capsid. Furthermore, the deletion or disruption of TRIM5 alpha(hu). domains necessary for potent N-MLV restriction completely abrogated the disappearance of particulate N-MLV capsids observed with wild-type TRIM5 alpha(hu). These results suggest that premature disassembly of the viral capsid contributes to the restriction of N-MLV infection by TRIM alpha(hu), but not by Fv-1.
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