Journal
EXPERIMENTAL PARASITOLOGY
Volume 115, Issue 3, Pages 310-313Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2006.09.008
Keywords
Plasmodium yoelii; transfection; malaria; green fluorescent protein
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Funding
- NIAID NIH HHS [5R01AI053698, R01 AI053698] Funding Source: Medline
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Plasmodium yoelii is a rodent parasite commonly used as a model to study malaria infection. It is the preferred model parasite for liverstage immunological studies and is also widely used to study hepatocyte, erythrocyte and mosquito infection. We have generated a P. yoelii yoelii 17XNL line that is stably transfected with the green fluorescent protein (gfp) gene. This parasite line constitutively expresses high levels of GFP during the complete parasite life cycle including liver, blood and mosquito stages. These fluorescent parasites can be used in combination with fluorescence activated cell sorting or live microscopy for a wide range of experimental applications. (c) 2006 Elsevier Inc. All rights reserved.
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