Journal
NATURE METHODS
Volume 4, Issue 3, Pages 223-225Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth1013
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We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a DNA molecule. We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form.
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