GPI-anchored CEA family glycoproteins CEA and CEACAM6 mediate their biological effects through enhanced integrin α5β1-fibronectin interaction

Carcinoembryonic antigen (CEA) and CEA family member CEACAM6 are glycophosphatidyl inositol (GPI)-anchored, intercellular adhesion molecules that are up-regulated in a wide variety of human cancers, including colon, breast, and lung. When overexpressed in a number of cellular systems, these molecules are capable of inhibiting cellular differentiation and anoikis, as well as disrupting cell polarization and tissue architecture, thus increasing tumorigenicity. The present study shows that perturbation of the major fibronectin receptor, integrin alpha 5 beta 1, underlies some of these biological effects. Using confocal microscopy and specific antibodies, CEA and CEACAM6 were demonstrated to co-cluster with integrin alpha 5 beta 1 on the cell Surface. The presence of CEA and CEACAM6 was shown to lead to an increase in the binding of the integrin alpha 5 beta 1 receptor to its ligand fibronectin, without changing its cell Surface levels, resulting in increased adhesion of CEA/CEACAM6-expressing cells to fibronectin. More tenacious binding of free fibronectin to cells led to enhanced fibronectin matrix assembly and the formation of a polymerized fibronectin cocoon around the cells. Disruption of this process with specific monoclonal antibodies against either fibronectin or integrin alpha 5 beta 1 led to the restoration of cellular differentiation and anoikis in CEA/CEACAM6 producing cells.