Journal
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
Volume 30, Issue 2, Pages 111-118Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cimid.2006.12.001
Keywords
isolation; salmonids; lactic acid bacteria; identification; DNA sequencing; 16S rRNA gene; variable regions
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The aim of this study was to identify lactic acid bacteria (LAB) using polymerase chain reaction (PCR) amplification of variable regions of the 16S rRNA gene. Thirteen LAB strains were isolated from the intestinal microbiota of healthy salmonids. A similar to 500-bp region of the highly conserved 16S rRNA gene was PCR-amplified and following this, a portion of the amplicon (272-bp) including the V1 and V2 variable regions was sequenced. The sequence containing both the V1 and V2 region provided strong evidence for the identification of LAB. The LAB strains were identified as Camobacteriton maltaroinaticum, Lactobacillus curvatus, Lactobacillus sakei, Lactobacillits plantarum, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, and Leuconostoc mesenteroides. The method described was found to be a very simple, rapid, specific, and low-cost tool for the identification of unknown strains of LAB. (c) 2007 Elsevier Ltd. All rights reserved.
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